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KMID : 1094720080130020182
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Biotechnology and Bioprocess Engineering
2008 Volume.13 No. 2 p.182 ~ p.188
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Pilot-scale production of carboxymethylcellulase from rice hull by Bacillus amyloliquefaciens DL-3
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Jo Kang-Ik
Lee You-Jung
Kim Bo-Kyung
Lee Bo-Hwa
Chung Chung-Han
Nam Soo-Wan
Kim Sung-Koo
Lee Jin-Woo
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Abstract
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Optimal conditions for pilot-scale production of the carboxymethylcellulase (CMCase) by Bacillus amyloliquefaciens DL-3 were investigated. The best carbon and nitrogen sources for the production of CMCase by B. amyloliquefaciens DL-3 were found to be rice hull and peptone and their optimal concentrations were 5.0 and 0.20% (w/v), respectively. Optimal temperature and initial pH for the production of CMCase were 37°C and 6.8. Optimal agitation speed and aeration rate for the production of CMCase were 300 rpm and 1.0 vvm in a 7 L bioreactor, which were different from those for the cell growth of B. amyloliquefaciens DL-3. The highest productions of CMCase by B. amyloliquefaciens DL-3 from 5.0% (w/v) rice hull as a carbon source under optimal conditions in a 7 or 100 L bioreactor were 220 and 367 U/mL, respectively.
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KEYWORD
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Bacillus amyloliquefaciens, carboxymethylcellulase (CMCase), optimization, rice hull
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